Coding

Part:BBa_K1869001

Designed by: Conary Meyer   Group: iGEM15_Santa_Clara   (2015-09-18)


SAM Synthetase

The S-adenosylmethionine (SAM) Synthetase is a cytosolic protein the is responsible for catalyzing the reaction between methionine and ATP to produce SAM. SAM functions as the primary methyl donor in numerous methylation reactions. It also plays a major role in in the SAM cycle and cysteine biosynthesis. This protein can be used in conjunction with other proteins to increase the concentration of methyl donors in a culture to help drive forward reactions. When used in conjunction with BBa_K1869000, the CFA synthase, SAM drives the CFA synthesis reaction forward.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 102
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 682
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None